The assay time is considerably reduced from about an hour to few minutes by the newly reported RTF-EXPAR method which uses reverse transcriptase-free (RTF) approach for conversion of RNA katika DNA followed by EXPAR (Exponential Amplification Reaction) for amplification at single temperature.
Controlling the rate of Covid-19 spread requires an accurate and a faster virus testing strategy. RT-PCR (reverse transcriptase polymerase chain reaction), the most accurate testing method being currently employed is a two-step test that takes around 60 minutes per sample.
Watafiti kutoka Chuo Kikuu cha Birmingham wameripoti njia ya riwaya ya kugundua SARS-CoV-2. Hii inaweza kuwezesha majaribio ya haraka zaidi na ni nyeti vya kutosha.
Utambuzi wa virusi vya RNA na RT-PCR (reverse transcriptase polymerase chain reaction) inahusisha ubadilishaji wa RNA ya virusi hadi DNA ya ziada (cDNA) ikifuatiwa na ukuzaji wa cDNA na PCR ya kiasi (qPCR). Kisha cDNA hugunduliwa kwa kutumia rangi ya fluorescent. Hii inachukua hadi saa moja.
The assay time is considerably reduced from about an hour to few minutes by the newly reported RTF-EXPAR method which uses reverse transcriptase-free (RTF) approach for conversion of RNA into DNA followed by EXPAR (Exponential Amplification Reaction) for amplification at single temperature. The amplification taking place at a single temperature is the key to speed, because it avoids lengthy heating and cooling steps of RT-PCR. Further, the section of DNA being amplified is smaller compared RT-PCR. Hence, EXPAR generates up to 108 strands of DNA product in few minutes. The duplex formation is monitored, as in RT-PCR method using fluorescent dye, SYBR Green.
Inashangaza, njia mpya inaweza kurekebishwa ili kugundua magonjwa mengine kadhaa ya kuambukiza yanayosababishwa na virusi vya RNA, kwa mfano Ebola, RSV, nk.
Chanzo (s):
Carter et al (2020). Utambuzi wa dakika 5 wa SARS-CoV-2 RNA kwa kutumia Mwitikio wa Ukuzaji wa Kielelezo wa Reverse Transcriptase-Free, RTF-EXPAR. Chapisha mapema. Iliyochapishwa katika medRxiv Iliyotumwa Januari 04, 2021. DOI: https://doi.org/10.1101/2020.12.31.20248236
***
